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Abstract
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Biochemical characterization of a custom expanded bed column made by the nanobiotechnology group (NBG) was compared with a commercial expanded bed contactor. Hydrodynamic characteristics of the columns (Daxl and Bo) with various settled bed heights along with a variety of column diameters were investigated. All parameters were found to be comparable or superior to those reported for conventional, expanded bed contactors. However, the experimental values of the Richardson–Zaki coefficient determined here were close to the value of 4.8, commonly used in the laminar flow regime. The expansion coefficient and terminal velocity of the adsorbent (Streamline DEAE) were theoretically calculated based on the literature and also experimentally determined. The adsorbent was also used in batch binding experiments, as well as in commercial and custom-assembled expanded bed contactors with various feedstocks comprising bovine serum albumin and egg albumin. This evaluation exhibited good capacities and adsorption/desorption performance of the NBG column in comparison with the commercial expanded bed column.
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