|
Abstract
|
As an innovative advancement in the realm of absolute quantitative polymerase chain
reaction methodologies, the droplet-based digital PCR (ddPCR) technique offers a
multitude of advantages including exceptionally high sensitivity, remarkable precision and
outstanding reproducibility, which are critical parameters in the realm of molecular
diagnostics. in light of the burgeoning demand for point-of-care (POC) detection and
clinical diagnostics, a low-cost, portable, and user-friendly droplet-based digital PCR
device has emerged as an intriguing focal point of research, attracting significant attention
from the scientific community. Given its extraordinary potential for seamless integration
and advanced miniaturization, microfluidic technology has been proficiently utilized across
a diverse range of digital droplet polymerase chain reaction (ddPCR) methodologies,
significantly augmenting both their operational efficiency and overall practicality in
various applications.
|