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Abstract
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The cultivated grapevine V. vinifera is a rich source of stilbene compounds such as resveratrol, which are widely
believed to provide dietary protection against the development of cardiovascular disease and some forms of
cancer. Elicitation is a well-known strategy to increase commercial production of natural products in plant cell
suspension culture systems. Callus tissues obtained from berry slices of V. vinifera cv. Shahani grown on an
optimized medium were used to develop cell suspension cultures used to study the effects of elicitation on
stilbene synthesis. The effect of two light regimes (135.1 μmol. s−1m−2 radiation, and dark), the concentration
of phenylalanine (Phe; 0, 0.1, 0.5 and 1 mM) and of methyl jasmonate elicitor (MeJA; 0 and 25 μM), alone or in
combination, were tested. The results showed that cultures grown in darkness resulted in significantly higher
levels of the accumulation of total stilbenes (resveratrol + piceid) compared with the high light condition. The
combined treatments of dark +1mM Phe and dark +25 μM MeJA induced the synthesis of high levels of total
phenolics, total flavonoids and total stilbenes. Finally, the combined elicitation of dark +1mM Phe+25 μM
MeJA gave the highest synergistic coefficient (1.24) and proved to be the most effective treatment for the
production of total phenolics, total flavonoids, and total stilbenes with mean contents of 384.80 mg GA/g DW,
527.62 mg catechin/g DW and 188.34 μg/g DW, respectively. The results of our study suggest that the combinations
of dark together with MeJA and/or Phe can be used as an efficient method for the future scale-up of V.
vinifera cell cultures for the production of high value stilbene compounds in a bioreactor system.
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