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Title
Molecular investigation of Canine parvovirus (CPV) antigenec variants from dogs with symptoms in Amol area.
Type Presentation
Keywords
canine parvovirus (CPV), dog, PCR, RFLP, antigenic variants
Abstract
Canine parvovirus type 2 (CPV-2) is one of the most hazardous canine diseases with rapid worldwide distribution causing seriously substantial hemorrhagic gastroenteritis disease. Depending on the severity of the disease and host immune status there is a wide range of symptoms that canines may show them. After that original CPV-2 was identified in the late 1970s, the new antigenic variants of this infectious virus have been identified steadily that are CPV-2a, CPV-2b, and CPV-2c. In this study, 20 rectal swab samples were investigated and analyzed by PCR methods and RFLP analysis using specific primers and MboII restriction enzyme, respectively. Our results indicated that 75% (15 out of 20) were CPV positive. In addition, 33.33% (5 out of 15) and 66.67% (10 out of 15) of positive cases were female and male, respectively. As well as, it was demonstrated that 66.67% (10 out of 15) and 33.33% (5 out of 15) positive cases were CPV-2b and CPV-2a, respectively by further research with PCR reaction and RFLP analysis. Additionally, CPV-2c was not detected in our study. These study revealed that CPV-2b has a higher spread in our area rather than other variants. Therefore, it seems the importance of further researches to introduce new vaccines containing all CPV variants is significant.
Researchers mojtaba khosravi (First researcher) , Ehsan Adiban (Second researcher)