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Keywords
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LINC01093, In-silico, Non-obstructive azoospermia, Gene expression, spermatogenesis
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Abstract
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Introduction: Numerous researches have recently focused on the function of Long noncoding RNAs (lncRNAs) in spermatogenesis and male infertility. Purpose: In the context of the etiopathogenesis of male infertility, we wanted to provide an overview of the information about the control of lncRNAs during spermatogenesis. Overall, the results are consistent with the theory that sperm abnormalities and infertility are linked to sperm lncRNAs. Material and Methods: Using Enrich Shiny GO, STRING, and Cytoscape online assessments, the functional and molecular interactions of proteins were predicted.
Results: A study published in this journal provides insight into the biological role of cytoskeleton proteins, such as DENN Domain Containing 5B (DENND5B), Zinc Finger Protein 587B (ZNF587B) and DCC netrin 1 receptor (DCC). A functional and molecular interactions prediction was performed using Enrich Shiny GO, STRING, and Cytoscape online evaluation in order to identify the master pathways between the proteins. According to the enrich tool analysis, DEGs that are up-regulated have three GO terms enriched in them, while DEGs that are down-regulated have three GO terms enriched. DEGs with up-regulation had three GO terms incorporated, while DEGs with down-regulation had three GO terms incorporated.
Discussion: Functional enrichment analysis demonstrated that the biological process (BP) term “Netrin receptor activity, Laminin-1 binding, Cysteine-type endopeptidase activity and Thiol-dependent deubiquitinate” was significantly overexpressed in DEGs. Over represented molecular function (MF) terms in up-regulated DEGs included “Axon guidance, Neuron projection guidance and Axon genesis”. According to our research, these genes and the hub proteins that they interact with may contribute to understanding the pathophysiology of aberrant germ cells and infertility
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