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Keywords
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Regenerative medicine, Tissue engineering, Bacterial cellulose scaffolds, Human mesenchymal stem cells, bone regeneration
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Abstract
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Natural extracellular matrixes (ECMs) such as bacterial cellulose have been recently absorbed
high attention as a novel biocompatible resource to replace animal derived scaffolds for human
stem cell culture which provide structural support for cell growth, attachments and
proliferation. Present work aimed to develop bacterial cellulose scaffold using different
compounds that are able to be produced in a sustainable way, as an alternative to animal sources
and evaluate their features after functionalization. We successfully could improve
hydrophilicity, surface roughness, chemical composition, surface morphology, porosity,
toxicity, swelling behavior and biodegradability as well as conductivity of the cellulosic
membrane that make it potential candidate for differentiation of human mesenchymal cells into
osteocytes confirmed by enhanced bone marker gene expression levels as well as higher
cellular mineralization, calcium secretion, and alkaline phosphatase (ALP) activity. It showed
that our modification can better mimic the structure and composition of the native ECM for
bone regeneration. Obtained results indicated that modified bacterial cellulose makes it an ideal
candidate towards the regeneration of the damaged bone tissues mainly due to the improved
properties such as enhanced wettability, and high tensile strength for future clinical applications
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