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Keywords
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anticancer activity, antioxidant, chitosan, iron oxide nanoparticle, silymarin
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Abstract
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Silymarin (SIL) contains a various flavonolignans extracted from Silybum marianum
and has an anti-cancer properties against a variety of cancers. In this study, two types
of improved nanocarriers based on iron oxide magnetic nanoparticles (MNPs) includ-ing chitosan anchored onto silica coated and uncoated Fe
3 O4
MNPs (Cs-f-SiO
2
@Fe 3 O4
MNPs and Cs-f -Fe
3 O4
MNPs) were successfully fabricated and charac-terized. Next, silymarin was loaded into the nanocarriers (SIL-Cs- f-SiO
2
@Fe 3O 4
MNPs and SIL-Cs-f- Fe
3 O4
MNPs). These systems were characterized and silymarin
content, antioxidant activity and intrinsic cytotoxicity were assessed. The func-tionalization degree of silymarin was assessed by the Folin–Ciocalteu method, finding
120 mg of SIL/g of Cs-f -SiO
2
@Fe 3 O4
MNPs and 99 mg of SIL/g of Cs-MNPs. The
antioxidant activity of the silymarin after loading into Cs- f- SiO
2
@Fe 3O 4
MNPs and
Cs-f- Fe
3O 4
MNPs was proved by DPPH method. These compounds have high anti-oxidant effect and their radical scavenging activity increased with increasing these
concentrations. SIL-Cs-f- SiO
2
@Fe 3O4
MNPs showed higher antioxidant activity than
the SIL-Cs-f -Fe
3 O4
MNPs in same concentrations. The cytotoxicity of Cs-f-SiO
2
@Fe 3 O4
MNPs and SIL-Cs-f- SiO
2
@Fe 3O 4
MNPs against MCF-7 was investi-gated by MTT assay and IC50 values were 110.03 and 73.56 μgml
1
respectively. In
conclusion, SIL-loaded MNPs can be used as drug delivery system on target in the
treatment of cancer cells.
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