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Abstract
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Campylobacter jejuni is one of the most important agents in gastrointestinal
infections both in developed and developing countries. Determining the
source of the human infections and the relationship of virulence gene
properties and origin of the isolates are necessary epidemiologic data to
control the infection in an area. We conducted a present study to genotype C.
jejuni isolates recovered from human, broiler, cattle, poultry meat, and cattle
meat in Mazandaran province, north of Iran. For this purpose, 100 isolates of
C. jejuni (20 isolates from each source), which had been previously isolated,
were subjected to Random Amplification of Polymorphic DNA Polymerase
Chain Reaction (RAPD-PCR), 16S rRNA Restriction Fragment Length
Polymorphism (RFLP) analysis and six virulence genes (cdtA, cdtB, cdtC,
cadF, iamA and pldA) pattern. In total, 98 isolates were typed using RAPDPCR
and 32 different genotypic patterns were observed. All 100 isolates
showed similar enzymatic digestion pattern in 16S rRNA-RFLP technique
by using DdeI and AluI enzymes. The cadF gene showed a 100% presence
among the isolates. The prevalence of cdtA, cdtB, cdtC, pldA, and iamA genes
was 97%, 97%, 96%, 72% and 60%, respectively. Ten different genotypic
patterns (VP1-VP10) were identified from the presence of six virulence
genes. According to the results, 16S rRNA-RFLP cannot type the isolates, but
both RAPD and virulence typing methods showed significant relationships
between some of the genotypes and a specific source. A potential role of
poultry isolates in human infections was observed using these two methods.
RAPD technique had the best results for typing of the Campylobacter isolates
between these three methods.
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