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Abstract
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Background Spermatogenesis is the process of producing mature sperm from Spermatogonial stem cells (SSCs)
and this process requires a complex cooperation of different types of somatic and germ cells. Undifferentiated
spermatogonia initiate the spermatogenesis and Sertoli cells as the only somatic cells inside of the seminiferous
tubule play a key role in providing chemical and physical requirements for normal spermatogenesis, here, we
investigated the dysfunction of these cells in non-obstructive azoospermia.
Material and method In this study, we analyzed the expression of sox9 and UTF1 in the non-obstructive human
testis by immunohistochemistry. Moreover, we used the KEGG pathway and bioinformatics analysis to reveal the
connection between our object genes and protein.
Results The immunohistochemistry analysis of the non-obstructive human seminiferous tubule showed
low expression of Sox9 and UTF1 that was detected out of the main location of the responsible cells for these
expressions. Our bioinformatics analysis clearly and strongly indicated the relation between UTF1 in undifferentiated
spermatogonia and Sox9 in Sertoli cells mediated by POU5F1.
Conclusion Generally, this study showed the negative effect of POU5F1 as a mediator between Sertoli cells as the
somatic cells within seminiferous tubules and undifferentiated spermatogonia as the spermatogenesis initiator germ
cells in non-obstructive conditions.
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