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Keywords
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POU5F1 (OCT4), spermatogonial stem cells, seminiferous tubules, analysis, transcription factor,
neonate, and adult germ cells
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Abstract
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POU5F1 (POU class 5 homeobox 1) is a transcription factor that is critically involved in the self-renewal of
undifferentiated embryonic stem cells. In this present study, we have developed our study to analyze the
expression of the POU5F1 in the neonatal and adult mice testis section and isolated spermatogonial stem cells
(SSCs). We also examine POU5F1 protein localization by three various kinds of antibodies. In this experimental
research, to enhance our understanding of the POU5F1 expression levels, protein localization, and
function in testicular germ cell, we used immunohistochemistry, immunocytochemistry, and Fluidigm real-time
polymerase chain reaction (RT-PCR) analysis in the mouse testis section and neonatal and adult SSCs, and also
we used protein-protein network analysis and gene enrichment analysis for genes involved in testicular development.
Counting POU5F1-positive cells represented significantly higher expression ( p < 0.05) of POU5F1
in the adult testis in comparison to the neonate. Finally, Fluidigm RT-PCR showed a significant expression
( p < 0.05) level of germ cells gene POU5F1 in neonate SSCs (1–2 week) than 16–24 week SSCs. The illustrated
results identify POU5F1 as a necessary transcription factor of testicular germ cells and can be supportive for the
investigation of the development and differentiation of SSCs.
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