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Abstract
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Glioblastoma (GB) is a common primary malignancy of the central nervous system, and one of the
highly lethal brain tumors. GB cells can promote therapeutic resistance and tumor angiogenesis. The
CD171 is an adhesion molecule in neuronal cells that is expressed in glioma cells as a regulator of
brain development during the embryonic period. CD171 is one of the immunoglobulin-like CAMs (cell
adhesion molecules) families that can be associated with prognosis in a variety of human tumors. The
multi-epitope peptide vaccines are based on synthetic peptides with a combination of both B-cell epitopes and T-cell epitopes, which can induce specific humoral or cellular immune responses. Moreover,
Cholera toxin subunit B (CTB), a novel TLR agonist was utilized in the final construct to polarize CD4þ
T cells toward T-helper 1 to induce strong cytotoxic T lymphocytes (CTL) responses. In the present
study, several immune-informatics tools were used for analyzing the CD171 sequence and studying
the important characteristics of a designed vaccine. The results included molecular docking, molecular
dynamics simulation, immune response simulation, prediction and validation of the secondary and tertiary structure, physicochemical properties, solubility, conservancy, toxicity as well as antigenicity and
allergenicity of the promising candidate for a vaccine against CD171. The immuno-informatic analyze
suggested 12 predicted multi-epitope peptides, whose construction consists of 582 residues long.
Therewith, cloning adaptation of the designed vaccine was performed, and eventually sequence
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