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Abstract
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In this study, the effect of solvent ratio of hexane to isopropanol (0:100, 50:50, and 100:0 v/v%), microwave power (180,
540, and 900 W) and extraction time (1, 2.5, and 4 min) were investigated on the oil extraction efficiency, total phenol
content, DPPH radical scavenging, peroxide value and oil color index. Extraction conditions were optimized by response
surface methodology and Box–Behnken Design. The optimal conditions were obtained as hexane-to-isopropanol ratio of
approximately 3:2, microwave power of 450 W and the extraction time of 1 min. Then, the hemp seed oil was extracted under
the optimal conditions. The optimal predicted contents for oil yield (26.34%), total phenolics (3.85 mg GA/g oil), DPPH
inhibition (74.29%), peroxide (3.94 meq/kg) and Color index (58.13) were agreed with the predicted conditions because the
Resident standard error (RSE) values were less than 5%.
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