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Abstract
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The aims of this study were to regain new epidemiology information about frequency, drug resistance rates, and
typing of Campylobacter jejuni (C. jejuni) isolates, obtained from some poultry and cattle farms, slaughterhouses,
and people with diarrhea. In this regard, Minimal Inhibitory Concentration (MIC) of several antibiotics and the
associated antibiotic resistance genes, including tetO, tetA, cmeB, and blaOXA-61 were evaluated. The isolates were
also typed, using the Fla-RFLP method. Generally, between 233 food animal samples, 80 (34.33%) C. jejuni were
isolated. Moreover, 20 out of 74 (27%) human specimens suspected to infectious diarrhea were C. jejuni positive.
High frequencies of resistance to tetracycline (100%), ciprofloxacin (95%), and nalidixic acid (86%), and low
frequencies of resistance to florfenicol (0%), erythromycin (5%), and gentamicin (8%) were observed.
Furthermore, in the tetracycline-resistant isolates, the existences of tetO, tetA, and cmeB were 86%, 23%, and
48%, respectively. There was a significant correlation between the cluster types obtained from Fla-RFLP method
and antibiotic resistance pattern. The results suggested that the genomic link between Campylobacter spp. should
be always evaluated in each country to provide an insight about the Campylobacter spp., spread in the region, in
order to implement the health-controlling programs efficiently.
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