مشخصات پژوهش

Introduction: One of the protein coding gene produced in spermatogenesis is late cornified envelope like proline rich 1 (LELP1), a zinc finger protein with a KRAB domain. LELP1 has a variety of functions, including killing of cells of other organism, defense response to gram-negative bacterium, and defense response to gram-positive bacterium. Material and methods: This research looked at two populations of human testicular cancer and normal spermatogonia to see how LELP1 was expressed. We examined LELP1 expression in vivo and in vitro by immunocytochemistry (ICC), immunohistochemistry (IMH), and Fluidigm real-time polymerase chain reaction. In addition, Enrich and Shiny Gene Ontology databases were used for pathway enrichment analysis and gene ontology. Results: According to IMH data, basal membrane had minimal LELP1 expression whereas the center of seminiferous tubules had a high concentration. The LELP1 antibody was positively expressed in the colonies of separated testicular cancer differentiated spermatogonia, according to ICC analysis, whereas LELP1 expression in the normal population was negative under in vitro conditions. LELP1 expression in testicular cancer differentiated spermatogonia was significantly higher than in healthy differentiated spermatogonia, according to Fluidigm real-time PCR analysis (p < 0.05). STRING and Cytoscape analyses presented seven genes, i.e., late cornified envelope 3C, Caspase-14, Involucrin, and Late cornified envelope protein 1F, as the hub genes involved in infertility with LELP1 co-function and protein–protein interaction. Conclusion: Our findings shown that LELP1 is an essential function in the differentiation stages of testicular germ cells and that LELP1 is increased in the spermatogenesis stages. These findings back up cutting-edge in vitro and in vivo studies of the spermatogenic process and cancer therapy.