مشخصات پژوهش

Natural extracellular matrixes (ECMs) such as bacterial cellulose have been recently absorbed high attention as a novel biocompatible resource to replace animal derived scaffolds for human stem cell culture which provide structural support for cell growth, attachments and proliferation. Present work aimed to develop bacterial cellulose scaffold using different compounds that are able to be produced in a sustainable way, as an alternative to animal sources and evaluate their features after functionalization. We successfully could improve hydrophilicity, surface roughness, chemical composition, surface morphology, porosity, toxicity, swelling behavior and biodegradability as well as conductivity of the cellulosic membrane that make it potential candidate for differentiation of human mesenchymal cells into osteocytes confirmed by enhanced bone marker gene expression levels as well as higher cellular mineralization, calcium secretion, and alkaline phosphatase (ALP) activity. It showed that our modification can better mimic the structure and composition of the native ECM for bone regeneration. Obtained results indicated that modified bacterial cellulose makes it an ideal candidate towards the regeneration of the damaged bone tissues mainly due to the improved properties such as enhanced wettability, and high tensile strength for future clinical applications